Abstract
Dispersible hydroxyapatite (HAp) nanoparticles are very useful for applying a monolayer to implantable medical devices using the nano-coating technique. To improve tolerance to infection on implanted medical devices, silver-doped HAp (Ag-HAp) nanoparticles with dispersiblity and crystallinity were synthesized, avoiding calcination-induced sintering, and evaluated for antibacterial activity.
The Ca10-xAgx(PO4)6(OH)2 with x = 0 and 0.2 were prepared by wet chemical processing at 100°C. Before calcination at 700°C for 2 h, two kinds of anti-sintering agents, namely a Ca(NO3)2 (Ca salt) and a polyacrylic acid/Ca salt mixture (PAA-Ca), were used. Escherichia coli was used to evaluate the antibacterial activity of the nanopowder.
When PAA-Ca was used as an anti-sintering agent in calcination to prepare the dispersible nanoparticles, strong metallic Ag peaks were observed at 38.1° and 44.3° (2θ) in the X-ray diffraction (XRD) profile. However, the Ag peak was barely observed when Ca salt was used alone as the anti-sintering agent. Thus, using Ca salt alone was more effective for preparation of dispersible Ag-HAp than PAA-Ca. The particle average size of Ag-HAp with 0.5 mol% of Ag content was found to be 325 ± 70 nm when the formation of large particle-aggregations was prevented, as determined by dynamic light scattering instrument. The antibacterial activity of the Ag-HAp nanoparticles possessing 0.5 mol% against
Dispersible and crystalline nano Ag-HAp can be obtained by using Ca salt alone as an anti-sintering agent. The nanoparticles showed antibacterial activity.
Int J Artif Organs 2015; 38(5): 251 - 258
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/ijao.5000408
Authors
Tsutomu Furuzono, Mazumder Motaharul, Yasumichi Kogai, Yoshinao Azuma, Yoshiki SawaArticle History
- • Accepted on 19/03/2015
- • Available online on 30/05/2015
- • Published in print on 17/06/2015
Disclosures
This article is available as full text PDF.
Authors
- Furuzono, Tsutomu [PubMed] [Google Scholar] 1, 2, * Corresponding Author ([email protected])
- Motaharul, Mazumder [PubMed] [Google Scholar] 1, 3
- Kogai, Yasumichi [PubMed] [Google Scholar] 3
- Azuma, Yoshinao [PubMed] [Google Scholar] 4
- Sawa, Yoshiki [PubMed] [Google Scholar] 2
Affiliations
-
Department of Biomaterial Engineering, School of Biology-Oriented Science and Technology, Kinki University, Wakayama - Japan -
Department of Surgery, Osaka University Graduate School of Medicine, Osaka - Japan -
Technical Development Center, SofSera Corporation, Osaka - Japan -
Department of Science and Technology on Food Safety, School of Biology-Oriented Science and Technology, Kinki University, Wakayama - Japan
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