Vol. 36 Issue 9 (September 2013)
Co-culture of human bone marrow stromal cells with endothelial cells alters gene expression profiles
Co-culture of human bone marrow stromal cells with endothelial cells alters gene expression profiles
Int J Artif Organs 2013; 36(9): 650 - 662
Article Type: ORIGINAL ARTICLE
DOI:10.5301/ijao.5000229
Authors
Ying Xue, Zhe Xing, Anne Isine Bolstad, Thomas E. Van Dyke, Kamal Mustafa
Abstract
The intricate relationship between angiogenesis and osteogenesis in vivo must be replicated in bone tissue engineering constructs to ensure the formation of a functional vascular network to support successful bone formation. Although communication between bone marrow stromal cells (MSC) and endothelial cells (EC) is recognized as one of the most important cellular interactions in bone regeneration, the underlying mechanisms of this biological process are not well understood. The purpose of this study was to analyze global gene expression associated with intercellular communication between MSC and EC using HumanWG-6 v3.0 expression BeadChips with a one-channel platform system (Illumina, San Diego, CA, USA). Each array contains more than 48,000 probes derived from human genes. A global map of MSC gene expression was generated following co-culture of MSC with EC for 5 and 15 days, in a direct-contact model. The map was used to determine relative alterations in functional processes and pathways. Co-culturing EC with MSC up-regulated genes related to angiogenesis as von Willebrand factor, platelet/endothelial cell adhesion molecule-1, cadherin 5, angiopoietin-related protein 4, and cell surface antigen CD34, and genes playing important roles in osteogenesis as alkaline phosphatase, FK506 binding protein 5, and bone morphogenetic protein. These findings clearly demonstrated that EC had a significant impact on MSC, particularly the bidirectional regulation of angiogenesis and osteogenesis. Moreover, cell-matrix interactions and TGF-β signal pathways were implicated for a crucial role in endothelial, cell-induced gene regulation in MSCs. A detailed study of the functional correlates of the microarray data is warranted to explore cellular and molecular interactions of importance in bone tissue engineering.
Article History
- • Accepted on 26/03/2013
- • Available online on 02/08/2013
- • Published in print on 03/10/2013
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Authors
- Xue, Ying
[PubMed]
[Google Scholar]
Department of Clinical Dentistry - Center for Clinical Dental Research, University of Bergen, Bergen - Norway
- Xing, Zhe
[PubMed]
[Google Scholar]
Department of Clinical Dentistry - Center for Clinical Dental Research, University of Bergen, Bergen - Norway
- Bolstad, Anne Isine
[PubMed]
[Google Scholar]
Department of Clinical Dentistry - Periodontics, University of Bergen, Bergen - Norway
- Dyke, Thomas E. Van
[PubMed]
[Google Scholar]
Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA - USA
- Mustafa, Kamal
[PubMed]
[Google Scholar]
Department of Clinical Dentistry - Center for Clinical Dental Research, University of Bergen, Bergen - Norway