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Design of artificial red blood cells using polymeric hydrogel microcapsules: hydrogel stability improvement and polymer selection

Abstract

Purpose

To improve the stability of pectin-oligochitosan hydrogel microcapsules under physiological conditions.

Methods

Two different approaches were examined: change of the cross-linker length and treatment of the hydrogel microcapsules with 150 Mm CaCl2. Replacement of pectin with alginate was also studied.

Results and Conclusions

It was observed that the molecular weight of the cross-linker oligochiotsan had no significant improvement on microcapsule stability. On the other hand, the treatment of pectin-oligochitosan microcapsules with Ca2+ increased the microcapsule stability significantly. Different types of alginate were used; however, no red-blood-cell-shaped microcapsules could be produced, which is likely due to the charge-density difference between deprotonated pectin and alginate polymers.

Int J Artif Organs 2016; 39(10): 518 - 523

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/ijao.5000532

Authors

Wujie Zhang, Matthew J. Bissen, Emily S. Savela, Joshua N. Clausen, Samantha J. Fredricks, Xiaoru Guo, Zachary R. Paquin, Ryan P. Dohn, Ian J. Pavelich, Alec L. Polovchak, Michael J. Wedemeyer, Brock E. Shilling, Emily N. Dufner, Anna C. O’Donnell, Gerardo Rubio, Logan R. Readnour, Tyler F. Brown, Jung C. Lee, Matey G. Kaltchev, Junhong Chen, Charles S. Tritt

Article History

Disclosures

Financial support: This work is supported by the Seed Money Grant from the Rader School of Business and the Faculty Summer Development Grant at the Milwaukee School of Engineering.
Conflict of interest: None of the authors has financial interest related to this study to disclose.

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Authors

  • Zhang, Wujie [PubMed] [Google Scholar] 1, * Corresponding Author ([email protected])
  • Bissen, Matthew J. [PubMed] [Google Scholar] 1
  • Savela, Emily S. [PubMed] [Google Scholar] 1
  • Clausen, Joshua N. [PubMed] [Google Scholar] 1
  • Fredricks, Samantha J. [PubMed] [Google Scholar] 1
  • Guo, Xiaoru [PubMed] [Google Scholar] 2
  • Paquin, Zachary R. [PubMed] [Google Scholar] 1
  • Dohn, Ryan P. [PubMed] [Google Scholar] 1
  • Pavelich, Ian J. [PubMed] [Google Scholar] 1
  • Polovchak, Alec L. [PubMed] [Google Scholar] 1
  • Wedemeyer, Michael J. [PubMed] [Google Scholar]
  • Shilling, Brock E. [PubMed] [Google Scholar] 1
  • Dufner, Emily N. [PubMed] [Google Scholar] 1
  • O’Donnell, Anna C. [PubMed] [Google Scholar] 1
  • Rubio, Gerardo [PubMed] [Google Scholar] 1
  • Readnour, Logan R. [PubMed] [Google Scholar]
  • Brown, Tyler F. [PubMed] [Google Scholar] 1
  • Lee, Jung C. [PubMed] [Google Scholar] 1
  • Kaltchev, Matey G. [PubMed] [Google Scholar] 1
  • Chen, Junhong [PubMed] [Google Scholar] 2, * Corresponding Author ([email protected])
  • Tritt, Charles S. [PubMed] [Google Scholar] 3

Affiliations

  • BioMolecular Engineering Program, Department of Physics and Chemistry, Milwaukee School of Engineering, Milwaukee, WI - USA
  • Department of Mechanical Engineering, University of Wisconsin-Milwaukee, Milwaukee, WI - USA
  • Biomedical Engineering Program, Department of Electrical Engineering and Computer Science, Milwaukee School of Engineering, Milwaukee, WI - USA

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